PRKAG1 (Center) Peptide
产品名称： PRKAG1 (Center) Peptide
英文名称： PRKAG1 (Center) Peptide
产品编号： P2145
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 所在区域 : 台湾
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PRKAG1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human PRKAG1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus.
Minokoshi Y, Alquier T, Furukawa N, Kim YB, Lee A, Xue B, Mu J, Foufelle F, Ferre P, Birnbaum MJ, Stuck BJ, Kahn BB.Nature. 2004 Apr 1;428(6982):569-74.
- 2.
- An activating mutation in the gamma1 subunit of the AMP-activated protein kinase.
Hamilton SR, Stapleton D, O'Donnell JB Jr, Kung JT, Dalal SR, Kemp BE, Witters LA.FEBS Lett. 2001 Jul 6;500(3):163-8.
- 3.
- Human immunodeficiency virus Tat protein induces interleukin 6 mRNA expression in human brain endothelial cells via protein kinase C- and cAMP-dependent protein kinase pathways.
Zidovetzki R, Wang JL, Chen P, Jeyaseelan R, Hofman F.AIDS Res Hum Retroviruses. 1998 Jul 1;14(10):825-33.
- Applications
- Western Blot (Tissue lysate)
- The PRKAG1 polyclonal antibody (Cat # PAB4579) is used in Western blot to detect PRKAG1 in mouse liver tissue lysate .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAG1 polyclonal antibody (Cat # PAB4579) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Entrez GeneID:
- 5571
- Protein Accession#:
- AAKG_HUMAN
- Gene Name:
- PRKAG1
- Gene Alias:
- AMPKG,MGC8666
- Gene Description:
- protein kinase, AMP-activated, gamma 1 non-catalytic subunit
- Omim ID:
- 602742
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
- Other Designations:
- 5'-AMP-activated protein kinase, gamma-1 subunit,AMP-activated protein kinase, noncatalytic gamma-1 subunit,AMPK gamma-1 chain